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A Validated Quantification of Sudan Red Dyes in Spicery using TLC and a 16-bit Flatbed Scanner
- We present a video-densitometric quantification method for Sudan red dyes in spices and spice mixtures, separated by TLC. Application was done band-wise in small dots using a 5 μL glass pipette. For separation, the RP-18 plates (20 × 20 cm with fluorescent dye; Merck, Germany, 1.05559) were developed in a vertical developing chamber without vapor saturation from the starting point to a distance ofWe present a video-densitometric quantification method for Sudan red dyes in spices and spice mixtures, separated by TLC. Application was done band-wise in small dots using a 5 μL glass pipette. For separation, the RP-18 plates (20 × 20 cm with fluorescent dye; Merck, Germany, 1.05559) were developed in a vertical developing chamber without vapor saturation from the starting point to a distance of 70 mm by using acetonitrile, methanol, and aqueous ammonia solution (25%; 8 + 1.8 + 0.2, v/v) as mobile phase. The quantification is based on direct measurements using an inexpensive 16-bit flatbed scanner for color measurements (in red, green, and blue). Evaluation of only the green channel makes the measurements very specific. For linearization, an extended Kubelka-Munk expression for data transformation was used. The range of linearity covers more than two magnitudes and lies between 20 and 500 ng. The extraction from a 2 g sample with acetonitrile, evaporation, and reconstitution to 200 μL with methanol and the band-wise application (7 mm) of a 10 μL sample allows a statistically defined LOD of less than 500 ppb of Sudan red dyes. To perform the analysis, a separation chamber, RP-18 plates, 5 μL glass pipettes, and a 16-bit flatbed scanner for 105 € are needed; therefore, the separation method is inexpensive, fast, and reliable.…
Document Type: | Article (reviewed) |
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Zitierlink: | https://opus.hs-offenburg.de/3144 | Bibliografische Angaben |
Title (English): | A Validated Quantification of Sudan Red Dyes in Spicery using TLC and a 16-bit Flatbed Scanner |
Author: | Barbara MilzStaff MemberGND, Philip Schnurr, Jannis GrafmüllerStaff MemberGND, Kevin Oehler, Bernd SpangenbergStaff MemberORCiDGND |
Year of Publication: | 2018 |
First Page: | 1397 |
Last Page: | 1401 |
Parent Title (English): | Journal of AOAC International |
Volume: | 101 |
Issue: | 5 |
ISSN: | 1060-3271 |
DOI: | https://doi.org/10.5740/jaoacint.18-0054 |
URL: | https://academic.oup.com/jaoac/article/101/5/1397/5654074 |
Language: | English | Inhaltliche Informationen |
Institutes: | Fakultät Maschinenbau und Verfahrenstechnik (M+V) |
Institutes: | Bibliografie | Formale Angaben |
Open Access: | Open Access |
Licence (German): | Urheberrechtlich geschützt |