A Simple and Reliable HPTLC Method for the Quantification of the Intense Sweetener Sucralose®
- This paper describes a simple and fast thin layer chromatography (TLC) method for the monitoring of the relatively new intense sweetener Sucralose® in various food matrices. The method requires little or no sample preparation to isolate or concentrate the analyte. The Sucralose® extract is separated on amino‐TLC‐plates, and the analyte is derivatized “reagent‐free” by heating the developed plateThis paper describes a simple and fast thin layer chromatography (TLC) method for the monitoring of the relatively new intense sweetener Sucralose® in various food matrices. The method requires little or no sample preparation to isolate or concentrate the analyte. The Sucralose® extract is separated on amino‐TLC‐plates, and the analyte is derivatized “reagent‐free” by heating the developed plate for 20 min at 190°C. Spots can be measured either in the absorption or fluorescence mode. The method allows the determination of Sucralose® at the levels of interest regarding foreseen European legislation (>50 mg/kg) with excellent repeatability (RSD = 3.4%) and recovery data (95%).…
Document Type: | Article (reviewed) |
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Zitierlink: | https://opus.hs-offenburg.de/4740 | Bibliografische Angaben |
Title (English): | A Simple and Reliable HPTLC Method for the Quantification of the Intense Sweetener Sucralose® |
Author: | Bernd SpangenbergStaff MemberORCiDGND, Jörg Stroka, Isabel Arranz, Elke Anklam |
Year of Publication: | 2003 |
Publisher: | Taylor & Francis |
First Page: | 2729 |
Last Page: | 2739 |
Parent Title (English): | Journal of Liquid Chromatography & Related Technologies |
Volume: | 26 |
Issue: | 16 |
ISSN: | 1082-6076 (Print) |
DOI: | https://doi.org/10.1081/JLC-120024543 |
URL: | https://www.tandfonline.com/doi/full/10.1081/JLC-120024543 |
Language: | English | Inhaltliche Informationen |
Institutes: | Fakultät Maschinenbau und Verfahrenstechnik (M+V) |
Institutes: | Bibliografie |
Tag: | Diode‐array TLC; Fluorescence detection; Fluorescence enhancement; HPTLC; Intense sweetener; Reagent‐free derivatization; Sucralose | Formale Angaben |
Open Access: | Closed Access |
Licence (German): | Urheberrechtlich geschützt |