Sensitive quantification of diclofenac and ibuprofen using thin layer chromatography coupled with a vibrio fisheri bioluminescence assay
- We present a video-densitometric quantification method for the pain killer known as diclofenac and ibuprofen. These non-steroidal anti-inflammatory drugs were separated on cyanopropyl bonded plates using CH2Cl2, methanol, cyclohexane (95 + 5 + 40, v/v) as mobile phase. The quantification is based on a bio-effective-linked analysis using Vibrio fisheri bacteria. Within 10 min a CCD-cameraWe present a video-densitometric quantification method for the pain killer known as diclofenac and ibuprofen. These non-steroidal anti-inflammatory drugs were separated on cyanopropyl bonded plates using CH2Cl2, methanol, cyclohexane (95 + 5 + 40, v/v) as mobile phase. The quantification is based on a bio-effective-linked analysis using Vibrio fisheri bacteria. Within 10 min a CCD-camera registered the white light of the light-emitting bacteria. Diclofenac and ibuprofen effectively suppressed the bacterial light emission which can be used for quantification within a linear range of 10 to 2000 ng. The detection limit for ibuprofen is 20 ng and the limit of quantification 26 ng per zone. Measurements were carried out using a 16-bit ST-1603ME CCD camera with 1.56 megapixels (from Santa Barbara Instrument Group, Inc., Santa Barbara, USA). The range of linearity covers more than two magnitudes because the extended Kubelka-Munk expression is used for data transformation. The separation method is inexpensive, fast, and reliable.…
Document Type: | Article (reviewed) |
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Zitierlink: | https://opus.hs-offenburg.de/3536 | Bibliografische Angaben |
Title (English): | Sensitive quantification of diclofenac and ibuprofen using thin layer chromatography coupled with a vibrio fisheri bioluminescence assay |
Author: | Andrea SeigelStaff Member, Alexandra Schröck, Rebekka Hauser, Bernd SpangenbergStaff MemberORCiDGND |
Date of Publication (online): | 2011/05/17 |
Publisher: | Taylor & Francis |
First Page: | 817 |
Last Page: | 828 |
Parent Title (English): | Journal of Liquid Chromatography & Related Technologies |
Volume: | 34 |
Issue: | 10/11 |
ISSN: | 1082-6076 |
DOI: | https://doi.org/10.1080/10826076.2011.571149 |
Language: | English | Inhaltliche Informationen |
Institutes: | Fakultät Maschinenbau und Verfahrenstechnik (M+V) |
Institutes: | Bibliografie |
GND Keyword: | Diclofenac; Dünnschichtchromatographie; Ibuprofen; Inhaltsstoff | Formale Angaben |
Open Access: | Closed Access |
Licence (German): | Urheberrechtlich geschützt |