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A simple and reliable method for quantification of glucosamine in nutritional supplements

  • HPTLC on amino plates, with simple heating of the plates for derivatization, has been used for quantification of glucosamine in nutritional supplements. On heating the plate glucosamine reacts to form a compound which strongly absorbs light between 305 and 330 nm, with weak fluorescence. The reaction product can be detected sensitively either by absorption of light or by fluorescence detection.HPTLC on amino plates, with simple heating of the plates for derivatization, has been used for quantification of glucosamine in nutritional supplements. On heating the plate glucosamine reacts to form a compound which strongly absorbs light between 305 and 330 nm, with weak fluorescence. The reaction product can be detected sensitively either by absorption of light or by fluorescence detection. The detection limit in absorption mode is approximately 25 ng per spot. In fluorescence mode a detection limit of 15 ng is achievable. A calibration plot for absorption detection is linear in the range 25 to 4000 ng glucosamine. The derivative formed from glucosamine by heating is stable for months, and the relative standard deviation is 1.64% for 600 ng glucosamine. The amounts of glucosamine found in nutritional supplements were in agreement with the label declarations.show moreshow less

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Metadaten
Author:Michaela Bleichert, Hanns Simon Eckhardt, Karl-Friedrich Klein, Bernd SpangenbergGND
Publisher:Akadémiai Kiadó
Year of Publication:2008
Language:English
GND Keyword:Ergänzung; Glucosamin; Nahrung
Parent Title (English):JPC - Journal of Planar Chromatography - Modern TLC
Volume:21
Issue:1
ISSN:0933-4173
First Page:55
Last Page:59
Document Type:Article (reviewed)
Institutes:Bibliografie
Acces Right:Frei zugänglich
Release Date:2019/10/22
Licence (German):License LogoEs gilt das UrhG
URL:https://www.researchgate.net/publication/245405290_A_simple_and_reliable_method_for_quantification_of_glucosamine_in_nutritional_supplements
DOI:https://doi.org/10.1556/JPC.2007.6005