New solvent systems to separate some estrogenically active compounds by high-performance thin-layer chromatography (HPTLC)
- Two solvent mixtures for high-performance thin-layer chromatographic (HPTLC) separation of some compounds showing estrogenic activity in the yeast estrogen screen (YES) assay are presented. The new method, planar yeast estrogen screen (pYES) combines the YES assay and a chromatographic separation on silica gel HPTLC plates with the performance of the YES assay. For separation, the analytes wereTwo solvent mixtures for high-performance thin-layer chromatographic (HPTLC) separation of some compounds showing estrogenic activity in the yeast estrogen screen (YES) assay are presented. The new method, planar yeast estrogen screen (pYES) combines the YES assay and a chromatographic separation on silica gel HPTLC plates with the performance of the YES assay. For separation, the analytes were applied bandwise to HPTLC plates (10 × 20 cm) with fluorescent dye (Merck, Germany). The plates were developed in a vertical developing chamber after 30 min of chamber saturation over a separation distance of 70 mm, using cyclohexane‒methyl-ethyl ketone (2:1, V/V) or cyclohexane‒CPME (3:2, V/V) as solvents. Both solvents allow separation of estriol, daidzein, genistein, 17β-estradiol, 17α-ethinyl estradiol, estrone, 4-nonylphenol and bis(2-ethylhexyl) phthalate.…
Document Type: | Article (reviewed) |
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Zitierlink: | https://opus.hs-offenburg.de/6547 | Bibliografische Angaben |
Title (English): | New solvent systems to separate some estrogenically active compounds by high-performance thin-layer chromatography (HPTLC) |
Author: | Bernd SpangenbergStaff MemberORCiDGND |
Year of Publication: | 2022 |
Publisher: | Springer International Publishing ; Akadémiai Kiadó |
First Page: | 189 |
Last Page: | 195 |
Parent Title (English): | JPC : Journal of Planar Chromatography - Modern TLC |
Volume: | 35 |
Issue: | 2 |
ISSN: | 1789-0993 (Online) |
ISSN: | 0933-4173 (Print) |
DOI: | https://doi.org/10.1007/s00764-022-00162-8 |
Language: | English | Inhaltliche Informationen |
Institutes: | Fakultät Maschinenbau und Verfahrenstechnik (M+V) |
Institutes: | Bibliografie |
Tag: | HPTLC | Formale Angaben |
Relevance: | Wiss. Zeitschriftenartikel reviewed: Listung in Master Journal List |
Open Access: | Open Access |
Hybrid | |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |