Screening of orange peel waste on valuable compounds by gradient multiple development diode-array high-performance thin-layer chromatography
- High-performance thin-layer chromatography (HPTLC), as the modern form of TLC (thin-layer chromatography), is suitable for detecting pharmaceutically active compounds over a wide polarity range using the gradient multiple development (GMD) technique. Diode-array detection (DAD) in conjunction with HPTLC can simultaneously acquire ultraviolet‒visible (UV‒VIS) and fluorescence spectra directly fromHigh-performance thin-layer chromatography (HPTLC), as the modern form of TLC (thin-layer chromatography), is suitable for detecting pharmaceutically active compounds over a wide polarity range using the gradient multiple development (GMD) technique. Diode-array detection (DAD) in conjunction with HPTLC can simultaneously acquire ultraviolet‒visible (UV‒VIS) and fluorescence spectra directly from the plate. Visualization as a contour plot helps to identify separated zones. An orange peel extract is used as an example to show how GMD‒DAD‒HPTLC in seven different developments with seven different solvents can provide an overview of the entire sample. More than 50 compounds in the extract can be separated on a 6-cm HPTLC plate. Such separations take place in the biologically inert stationary phase of HPTLC, making it a suitable method for effect-directed analysis (EDA). HPTLC‒EDA can even be performed with living organism, as confirmed by the use of Aliivibrio fischeri bacteria to detect bioluminescence as a measure of toxicity. The combining of gradient multiple development planar chromatography with diode-array detection and effect-directed analysis (GMD‒DAD‒HPTLC‒EDA) in conjunction with specific staining methods and time-of-flight mass spectrometry (TOF‒MS) will be the method of choice to find new chemical structures from plant extracts that can serve as the basic structure for new pharmaceutically active compounds.…
Document Type: | Article (reviewed) |
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Zitierlink: | https://opus.hs-offenburg.de/6548 | Bibliografische Angaben |
Title (English): | Screening of orange peel waste on valuable compounds by gradient multiple development diode-array high-performance thin-layer chromatography |
Author: | Bernd SpangenbergStaff MemberORCiDGND, Andrea SeigelStaff Member, Regina Brämer Staff Member |
Year of Publication: | 2022 |
Publisher: | Springer ; Akadémiai Kiadó |
First Page: | 313 |
Last Page: | 330 |
Parent Title (English): | JPC : Journal of Planar Chromatography - Modern TLC |
Volume: | 35 |
Issue: | 3 |
ISSN: | 1789-0993 (Online) |
ISSN: | 0933-4173 (Print) |
DOI: | https://doi.org/10.1007/s00764-022-00176-2 |
Language: | English | Inhaltliche Informationen |
Institutes: | Fakultät Maschinenbau und Verfahrenstechnik (M+V) |
Institutes: | Bibliografie |
Tag: | HPTLC | Formale Angaben |
Relevance: | Wiss. Zeitschriftenartikel reviewed: Listung in Master Journal List |
Open Access: | Open Access |
Hybrid | |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |