The Mineralization of Various 3D-Printed PCL Composites
- In this project, different calcification methods for collagen and collagen coatings were compared in terms of their applicability for 3D printing and production of collagen-coated scaffolds. For this purpose, scaffolds were printed from polycaprolactone PCL using the EnvisionTec 3D Bioplotter and then coated with collagen. Four different coating methods were then applied: hydroxyapatite (HA)In this project, different calcification methods for collagen and collagen coatings were compared in terms of their applicability for 3D printing and production of collagen-coated scaffolds. For this purpose, scaffolds were printed from polycaprolactone PCL using the EnvisionTec 3D Bioplotter and then coated with collagen. Four different coating methods were then applied: hydroxyapatite (HA) powder directly in the collagen coating, incubation in 10× SBF, coating with alkaline phosphatase (ALP), and coating with poly-L-aspartic acid. The results were compared by ESEM, µCT, TEM, and EDX. HA directly in the collagen solution resulted in a pH change and thus an increase in viscosity, leading to clumping on the scaffolds. As a function of incubation time in 10× SBF as well as in ALP, HA layer thickness increased, while no coating on the collagen layer was apparently observed with poly-L-aspartic acid. Only ultrathin sections and TEM with SuperEDX detected nano crystalline HA in the collagen layer. Exclusively the incubation in poly-L-aspartic acid led to HA crystals within the collagen coating compared to all other methods where the HA layers formed in different forms only at the collagen layer.…
Document Type: | Article (reviewed) |
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Zitierlink: | https://opus.hs-offenburg.de/6288 | Bibliografische Angaben |
Title (English): | The Mineralization of Various 3D-Printed PCL Composites |
Author: | Artem Egorov, Bianca Riedel, Johannes VinkeStaff MemberORCiDGND, Hagen Schmal, Ralf Thomann, Yi Thomann, Michael Seidenstuecker |
Year of Publication: | 2022 |
Date of first Publication: | 2022/11/11 |
Place of publication: | Basel |
Publisher: | MDPI |
First Page: | 1 |
Last Page: | 18 |
Article Number: | 238 |
Parent Title (English): | Journal of Functional Biomaterials |
Editor: | Sandra Varnaitė-Žuravliova, Jolanta Sereikaitė, Julija Baltušnikaitė-Guzaitienė |
Volume: | 13 |
Issue: | 4 |
ISSN: | 2079-4983 |
DOI: | https://doi.org/10.3390/jfb13040238 |
URN: | https://urn:nbn:de:bsz:ofb1-opus4-62883 |
Language: | English | Inhaltliche Informationen |
Institutes: | Fakultät Maschinenbau und Verfahrenstechnik (M+V) |
Institutes: | Bibliografie |
Tag: | 3D printing; PCL scaffolds; alkaline phosphatase; collagen coating; hydroxyapatite; poly-L aspartic acid | Formale Angaben |
Relevance: | Wiss. Zeitschriftenartikel reviewed: Listung in Master Journal List |
Open Access: | Open Access |
Gold | |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |